J. Griffié, G.L. Burn, D.J. Williamson, P. Rubin-Delanchy and D.M. Owen. Dynamic Bayesian cluster analysis of live-cell SMLM datasets. Small Methods (accepted).
G.W. Ashdown, D.J. Williamson, G. Soh, N. Day, G.L. Burn and D.M. Owen. Membrane lipid order of sub-synaptic T cell vesicles correlates with their dynamics and function. Traffic 19, 29-35 (2018).
S. Simoncelli, M. Makarova, W. Wardley, and D.M. Owen. Toward an axial nanoscale ruler for fluorescence microscopy. ACS Nano 11(12) 11762-11767 (2017). (invited perspective).
J. Griffié, L. Shlomovich, D.J. Williamson, M.J. Shannon, J. Aaron, S. Khuon, G.L. Burn, L. Boelen, R. Peters, A.P. Cope, E.A.K. Cohen, P. Rubin-Delanchy and D.M. Owen. 3D Bayesian cluster analysis of super-resolution iPALM data reveals the recruitment of LAT vesicles to the T cell immunological synapse. Scientific Reports 7(4077), 1-9 (2017).
R. Peters, M. Bentham-Muňiz, J. Griffié, D.J. Williamson, G.W. Ashdown, C.D. Lorenz and D.M. Owen. Quantification of fibrous spatial point patterns from single-molecule localisation microscopy (SMLM) data. Bioinformatics 33(11), 1703-1711 (2017).
G.W. Ashdown, G.L. Burn, E. Pandžić, R. Peters, M. Holden, H. Ewers, L. Shao, P.W. Wiseman and D.M. Owen. Live-cell super-resolution reveals the architecture, dynamics and coupling of F-actin and the plasma membrane at the T cell immunological synapse. Biophysical Journal 112(8), 1703-1713 (2017).
O.O. Glebov, R. Jackson, C.M. Winterflood, D.M. Owen, E.A. Barker, P. Doherty, H. Ewers and J. Burrone. Nanoscale structural plasticity of the active zone matrix modulates presynaptic function. Cell Reports 18(11), 2715-2728 (2017).
N.S. Poulter, A.Y. Pollitt, D.M. Owen, E.E. Gardiner, R.K. Andrews. H. Shimizu, D. Ishikawa, D. Bihan, R.W. Farndale, M. Moroi, S.P. Watson and S.M. Jung. Receptor clustering as a mechanism to regulate GPVI signalling in platelets. Journal of Thrombosis and Haemostasis 15, 549-564 (2017).
E. Kemal, T.F. Abelha, L. Urbano, R. Peters, D.M. Owen, M. Green and L.-A. Daily. Bright, Near Infrared Emitting PLGA-PEG-Capped Dye-Doped CN-PPV Nanoparticles for Imaging Applications. RSC Advances 7, 15255-15264 (2017).
P.R. Nicovich, D.M. Owen and K. Gaus. Quantitative analysis of pointillist single molecule localisation microscopy data sets. Nature Protocols 12(3), 453-461 (2017).
J. Griffié, M.J. Shannon, C.L. Bromley, L. Boelen, G.L. Burn, D.J. Williamson, N. Heard, A.P. Cope, D.M. Owen* and P. Rubin-Delanchy*. Bayesian cluster analysis of single molecule localisation microscopy data. Nature Protocols 11, 2499-2514 (2016). *Equal senior author.
R. Peters, L. Sandiford, D.M. Owen, E. Kemal, S. Burke, L.-A. Daily and M. Green. Red-emitting protein-coated conjugated polymer nanoparticles. Photochemical and Photobiological Sciences 15. 1448-1452 (2016).
G.L. Burn, G.H. Cornish, K. Potrzebowska, M. Samuelsson, J. Griffié, S. Minoughan, M. Yates, G.W. Ashdown, N. Pernodet, V.L. Morrison, C. Sanchez-Blanco, H. Purvis, F. Clarke, R.J. Brownlie, T. Vyse, R. Zamoyska, D.M. Owen*, L.M. Svensson* and A.P. Cope*. Super-resolution imaging of the cytoplasmic phosphatase PTPN22 links integrin-mediated T cell adhesion with autoimmunity. Science Signaling 9, ra99 (2016). *Equal senior author
N. Gustafsson, S. Culley, G.W. Ashdown, D.M. Owen, P.M. Pereira and R. Henriques. Fast live-cell conventional fluorophore nanoscopy with ImageJ through super-resolution radial fluctuations. Nature Communications 7, 12471 (2016).
S. Taniguchi, L. Sandiford, M. Cooper, E.V. Rosca, R.A. Khanbeigi, S.M. Fairclough, M. Thanou, L.-A. Dailey, W. Wohlleben, B. von Vacano, R.T.M. de Rosales, P.J. Dobson, D.M. Owen and M. Green. Hydrophobin-encapsulated quantum dots. ACS Applied Materials and Interfaces 8(7) 4887-4893 (2016).
A. Abu-Siniyeh, D.M. Owen, C. Benzing, S. Rinkwitz, T.S. Becker, A. Majumdar and K. Gaus. The aPKC/Par3/Par6 polarity complex and membrane order are functionally inter‐dependent in epithelia during vertebrate organogenesis. Traffic 17(1), 66-79 (2016). *including cover art.
G.W. Ashdown, E. Pandžić, A.P. Cope, P.W. Wiseman and D.M. Owen. Measuring molecular flow in super-resolution fluorescence microscopy. Journal of Visual Experiments 106, e53749 (2015).
P. Rubin-Delanchy, G.L. Burn, J. Griffié, D.J. Williamson, N. Heard, A.P. Cope and D.M. Owen. Bayesian cluster identification in single-molecule localisation microscopy data. Nature Methods 12(11), 1072-1076 (2015).
J. Griffié, L. Boelen, G.L. Burn, A.P. Cope and D.M. Owen. Topological prominence as a tool for cluster identification in single-molecule localisation data. Journal of Biophotonics 8(11), 925-934 (2015).
X. Cheng, E. Hinde, D.M. Owen, S.B. Lowe, P.J. Reece, K. Gaus and J.J. Gooding. Enhancing quantum dots for bioimaging using advanced surface chemistry and advanced optical microscopy: application to silicon quantum dots (SiQDs). Advanced Materials 27(40), 6144-6150 (2015).
A. Magenau, D.M. Owen, Y. Yamamoto, J. Tran, J.M. Kwiatek, R.G. Parton and K. Gaus. Discreet and distinct clustering of five model membrane proteins revealed by single molecule localization microscopy. Molecular Membrane Biology 32(1), 11-18 (2015).
N.S. Poulter, A.Y. Pollitt, A. Davies, D. Malinova, G.B. Nash, M.J. Hannon, Z. Pikramenou, J.Z. Rappoport, J.H. Hartwig, D.M. Owen, A.J. Thrasher, S.P. Watson and S.G. Thomas. Platelet actin nodules are podosome-like structures dependent on Wiskott-Aldrich Syndrome protein and Arp2/3 complex. Nature Communications 6, 7254 (2015).
M.J. Shannon, G.L. Burn, A.P. Cope, G. Cornish* and D.M. Owen*. Application of Super-resolution microscopy to the analysis of protein clustering in T cells. Biochemical Society Transactions 43(3), 315-321 (2015) *Equal senior author.
A. Alvarez-Guaita, S. Vilà de Muga, D.M. Owen, D.J. Williamson, A. Magenau, A. García-Melero, M. Reverter, M. Hoque, R. Cairns, R. Cornely, F. Tebar, T. Grewal, K. Gaus, J. Ayala-Sanmartin, C. Enrich and C. Rentero. Evidence for Annexin A6-dependent plasma membrane remodelling of lipid domains. British Journal of Pharmacology 172(7), 1677-1690 (2015).
A. Le Marois, D.M. Owen and K. Suhling. Investigating cell membrane structure and dynamics using TCSPC-FLIM. Proceedings of the Society of Photo-Optical Instrumentation Engineers (SPIE) 9329(38), 1-7 (2015).
A.Y. Pollitt, N.S. Poulter, E. Gitz, L. Navarro-Nuñez, Y.-J. Wang, C.E. Hughes, S.G. Thomas, B. Nieswandt, M.R. Douglas, D.M. Owen, D.G. Jackson, M.L. Dustin and S.P. Watson. Syk and Src family kinases regulate C-type lectin (CLEC-2)-mediated clustering of Podoplanin and platelet adhesion to lymphatic endothelial cells. Journal of Biological Chemistry 289(52), 35695-35710 (2014).
C.T. Bach, R Murray, D.M. Owen, K. Gaus and G.M. O’Neill. Tropomyosin Tm5NM1 spatially restricts Src kinase activity through perturbation of Rab11 vesicle trafficking. Molecular and Cellular Biology 34(24), 4436-4446 (2014).
G.W. Ashdown, A.P. Cope, P.W. Wiseman and D.M. Owen. Molecular flow quantified beyond the diffraction limit by spatio-temporal image correlation of structured illumination microscopy data. Biophysical Journal 107(9), L21-L23 (2014).
J. Rossy, E. Cohen, K. Gaus and D.M. Owen. Method for co-clustering analysis in multi-channel single-molecule localisation data. Histochemistry and Cell Biology 141, 605-612 (2014).
D.M. Owen and K. Gaus. Imaging lipid domains in cell membranes: The advent of super-resolution fluorescence microscopy. Frontiers in Plant Science 4(503), 1-9 (2013).
D.M. Owen, D.J. Williamson, L. Boelen, A. Magenau, J. Rossy and K. Gaus. Quantitative analysis of 3-dimensional fluorescence localisation microscopy data. Biophysical Journal 105(2), L05-L07 (2013).
S.V. Pageon, S.-P. Cordoba, D.M. Owen, S.M. Rothery and D.M. Davis. Super-resolution microscopy reveals the nanometre-scale reorganisation of inhibitory NK cell receptors following ligation of the activating receptor NKG2D*. Science Signaling** 6(285), ra62 (2013).*highlighted in Nature (2013) **Including cover art
R. Rollason, P. Billcliff, I. Prior, D.M. Owen, K. Gaus and G. Banting. CD317/Tetherin is an organiser of membrane microdomains. Journal of Cell Science 126(7), 1553-1564 (2013).
D. Karanakaran, M. Kockx, D.M. Owen, J.R. Burnett, W. Jessup and L. Kritharides. Protein Kinase C controls vesicular transport and secretion of apolipoprotein E from primary human macrophages. Journal of Biological Chemistry 288(7), 5186-5197 (2013).
J.M. Kwiatek,* D.M. Owen,* A. Abu-Siniyeh, P. Yan, L.M. Loew and K. Gaus. Characterization of a new generation of fluorescent probes for imaging membrane order. PLoS ONE 8(2), e52960 (2013). *Equal first author
J. Rossy, D.M. Owen, D.J. Williamson, Z. Yang and K. Gaus. Conformational states of the kinase Lck regulate clustering in early T cell signalling. Nature Immunology 14(1), 82-89 (2013).
D.M. Owen, D.J. Williamson, A. Magenau and K. Gaus. Sub-resolution lipid domains exist in the plasma membrane and regulate protein diffusion and distribution. Nature Communications 3, 1256 (2012).
M. Rees, L. Dang, T. Thai, D.M. Owen, E. Malle and S.R. Thomas. Targeted Subendothelial Matrix Oxidation by Myeloperoxidase Triggers Myosin II-Dependent De-adhesion and Alters Signaling in Endothelial Cells. Free Radical Biology and Medicine, 53(12) 2344-2356 (2012).
D. Svistounov, A. Warren, G.P. McNerney, D.M. Owen, D. Zencak, S. Zykova, H. Crane, T. Huser, R.J. Quinn, B. Smedsrød, D.G. Le Couteur and V.C. Cogger. The relationship between fenestrations, sieve plates and rafts in liver sinusoidal endothelial cells. PLoS ONE 7(9), e46134 (2012).
D.M. Owen, A. Magenau, D.J. Williamson and K. Gaus. The lipid raft hypothesis revisited: New insights from new microscopy techniques. BioEssays 34(9), 739-747 (2012).
D.M. Owen, M. Sauer and K. Gaus. Fluorescence localisation microscopy – the transformation from concept to biological research tool. Communicative and Integrative Biology 5(4), 345-349 (2012).
C.F. Pereira*, J. Rossy*, D.M. Owen, K. Gaus† and J. Mak†. HIV taken by STORM: Super-resolution fluorescence microscopy of a viral infection. Virology Journal 9(1), 84-90 (2012). †*Equal first/last author
T. Nomura, C.G. Cranfield, E. Deplazes, D.M. Owen, A.M. Macmillan, A.R. Battle, M. Sokabe and B. Martinac. Lipid and lyso-lipid effects on the mechanosensitivity of MscL and MscS co-reconstituted into liposomes. Proceedings of the National Academy of Sciences (PNAS) 109(22), 8770-8775 (2012).
D.M. Owen, C. Rentero, A. Abu-Siniyeh, A. Magenau and K. Gaus. Imaging membrane order using Laurdan and di-4-ANEPPDHQ in live cells and whole organisms. Nature Protocols 7(1), 24-35 (2012).
D.J. Williamson*, D.M. Owen*, J. Rossy*, A. Magenau, M. Wehrmann, J.J. Gooding and K. Gaus. Pre-existing LAT clusters do not participate in early T cell signaling events. Nature Immunology 12(7), 655-662 (2011). *Equal first author
S. Dehn, K.W.K. Tong, R.G.C. Clady, D.M. Owen, K. Gaus, T.W. Schmidt, F. Braet and P. Thordarson. The Structure and Luminescence Properties of Europium(III) Triflate Doped Self-assembled Pyromellitamide Gels. New Journal of Chemistry 35(7), 1466-1471 (2011).
L. Miguel, D.M. Owen, C. Leibig, C. Lim, J. Evans, A.I. Magee and E.C. Jury. Primary human CD4+ T cells have diverse levels of membrane lipid order that correlate with their function. Journal of Immunology 186(6), 3505-3516 (2011).
D.M. Owen, A.I. Magee, K. Gaus and M. Cebecauer. Dynamic organisation of lymphocyte plasma membranes: Lessons from advanced imaging methods. Immunology 131, 1-8 (2010).
D.M. Owen, S. Oddos, S. Kumar, D.M. Davis, M.A.A. Neil, P.M.W. French, M.L. Dustin, A.I. Magee and M. Cebecauer. High plasma membrane order imaged at the immunological synapse periphery in live T cells. Molecular Membrane Biology 27(4-6), 178-189 (2010).
D.M. Owen, A. Magenau, A. Majumdar and K. Gaus. Imaging membrane lipid order in whole, living vertebrate organisms*. Biophysical Journal 99(1), L7-L9 (2010). *Highlighted in Nature (2010)
M.A. Purbhoo, H.B. Liu, S. Oddos, D.M. Owen, M.A.A. Neil, S.V. Pageon, P.M.W. French, C.E. Rudd and D.M. Davis. Dynamics of sub-synaptic vesicles and surface microclusters at the immunological synapse. Science Signaling 3(121), ra36 1-10 (2010).
D.M. Owen, C. Rentero, J. Rossy, A. Magenau, D.J. Williamson, M. Rodriguez and K. Gaus. PALM imaging and cluster analysis of protein heterogeneity at the cell surface. Journal of Biophotonics 3(7), 446-454 (2010).
D.M. Owen and K. Gaus. Optimized time-gated generalized polarization (TGGP) imaging of Laurdan and di-4-ANEPPDHQ for membrane order image contrast enhancement. Microscopy Research and Technique 73(6), 618-622 (2010).
M. Cebecauer, D.M. Owen, A. Markiewicz and A.I. Magee. Lipid order and molecular assemblies in the plasma membrane of eukaryotic cells. Biochemical Society Transactions 37(5), 1056-1060 (2009).
D.M. Owen, D.J. Williamson, C. Rentero and K. Gaus. Quantitative microscopy: Protein dynamics and membrane organization. Traffic 10(8), 962-671 (2009).
C.B. Talbot, J. McGinty, D.M. Grant, E.J. McGhee, D.M. Owen, W. Zhang, T.D. Bunney, I. Munro, B. Isherwood, R. Eagle, A. Hargreaves, M. Katan, A.I. Magee, C.W. Dunsby, M.A.A. Neil and P.M.W. French. High-speed unsupervised fluorescence lifetime imaging confocal multiwell plate reader for high content analysis. Journal of Biophotonics 1(6), 514-521 (2008).
H.B. Manning, G.T. Kennedy, D.M. Owen, D.M. Grant, M. Katan, A.I. Magee, M.A.A. Neil, C.W. Dunsby, Y. Itoh and P.M.W. French. A compact, multidimensional spectrofluorometer exploiting supercontinuum generation. Journal of Biophotonics 1(6), 494-505 (2008).
S. Oddos, C.W. Dunsby, M.A. Purbhoo, A. Chauveau, D.M. Owen, M.A.A. Neil, D.M. Davis and P.M.W. French. High-speed, high-resolution imaging of intracellular immune synapses using optical tweezers. Biophysical Journal 95(10), L66-L68 (2008).
T. Robinson, P. Valluri, H.B. Manning, D.M. Owen, I. Munro, C.B. Talbot, C.W. Dunsby, J.F. Eccleston, G.S. Baldwin, M.A.A. Neil, A.J. de Mello and P.M.W. French. Three-dimensional molecular mapping in a microfluidic mixing device using fluorescence lifetime imaging. Optics Letters 33(16), 1887-1889 (2008).
V. Poher, G.T. Kennedy, H.B. Manning, D.M. Owen, H.X. Zhang, E. Gu, M.D. Dawson, P.M.W. French and M.A.A. Neil. Method of improving axial resolution and image contrast in a slit scanning confocal microscope. Optics Letters 33(16), 1813-1815 (2008).
D.M. Owen, E. Auksorius, H.B. Manning, C.B. Talbot, P.A.A. de Beule, C.W. Dunsby, M.A.A. Neil and P.M.W. French. Excitation-resolved hyperspectral fluorescence lifetime imaging using a UV-extended supercontinuum source. Optics Letters 32(23), 3408-3410 (2007).
D.M. Grant, J. McGinty, E.J. McGhee, T.D. Bunney, D.M. Owen, C.B. Talbot, W. Zhang, S. Kumar, I. Munro, P.M.P. Lanigan, G.T. Kennedy, C.W. Dunsby, A.I. Magee, P. Courtney, M. Katan, M.A.A. Neil and P.M.W. French. High speed optically-sectioned fluorescence lifetime imaging permits study of live cell signaling events. Optics Express 15(24), 15656-15673 (2007).
D.M. Owen, M.A.A. Neil, P.M.W. French and A.I. Magee. Optical techniques for imaging membrane microdomains in living cells. Seminars in Cell and Developmental Biology 18(5), 591-598 (2007).
S. Kumar, C.W. Dunsby, P.A.A. de Beule, D.M. Owen, U. Anand, P.M.P. Lanigan, R.K.P. Benninger, D.M. Davis, M.A.A. Neil, P. Anand, C. Benham, A. Naylor and P.M.W. French. Multifocal and multiphoton excitation and time correlated single photon counting detection for 3-D fluorescence lifetime imaging. Optics Express 15(20), 12548-12561 (2007).
V.S. Lopes, J.S. Ramalho, D.M. Owen, M.O. Karl, O. Strauss, C.E. Futter and M.C. Seabra. The ternary Rab27a:Myrip:MyosinVIIa complex regulates melanosome motility in the retinal pigment epithelium. Traffic 8(5), 486-499 (2007).
P.A.A. de Beule, D.M. Owen, H.B. Manning, C.B. Talbot, J. Requejo-Isidro, C.W. Dunsby, J. McGinty, R.K.P. Benninger, D.S. Elson, I. Munro, J. Lever, P. Anand, M.A.A. Neil and P.M.W. French. Rapid hyperspectral fluorescence lifetime imaging. Microscopy Research and Technique 70(5), 481-484 (2007).
H.B. Manning, D.M. Owen, E. Auksorius, S. Oddos, P.A.A. de Beule, C.B. Talbot, A.I. Magee, M.A.A. Neil and P.M.W. French. Applications of rapid, time-gated hyperspectral FLIM: Live cell imaging of membrane order and 6-D microscopy. Proceedings of the Society of Photo-Optical Instrumentation Engineers (SPIE) 6630, 663019-1-663019-12 (2007).
D.M. Owen, H.B. Manning, P.A.A. de Beule, C.B. Talbot, J. Requejo-Isidro, C.W. Dunsby, J. McGinty, R.K.P. Benninger, D.S. Elson, I. Munro, N.P. Galletly, J. Lever, G.W. Stamp, P. Anand, M.A.A. Neil and P.M.W. French. Development of a hyperspectral fluorescence lifetime imaging microscope and its application to tissue imaging. Proceedings of the Society of Photo-Optical Instrumentation Engineers (SPIE) 6441, K1-K9 (2007).
P.A.A. De Beule, C.W. Dunsby, D.M. Owen, N.P. Galletly, U. Anand, C.D. Benham, A. Naylor, G.W. Stamp, P. Anand and P.M.W. French. A novel hyperspectral lifetime probe for autofluorescence. Proceedings of the Society of Photo-Optical Instrumentation Engineers (SPIE) 6433, 3-9 (2007).
D.M. Owen, P.M.P. Lanigan, C.W. Dunsby, I. Munro, D.M. Grant, M.A.A. Neil, P.M.W. French and A.I. Magee. Fluorescence lifetime imaging provides enhanced contrast when imaging the phase-sensitive dye di-4-ANEPPDHQ in model membranes and live cells. Biophysical Journal 90(11), L80-L82 (2006).